Purified Anti-Rat CD3 Antibody[G4.18], Functional Grade (E-AB-F12280)
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For research use only.
| Alternate Names | Cd3g |
| Clone | |
| Verified Samples | Verified Samples in FCM: Rat splenocytes |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume;FA Reported in the literature;Activ Reported in the literature |
| Background | CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Rat CD3 protein |
| Abbre | CD3 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Rat |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG3, κ |
| Applications |
FCM;FA;Activ
|
| Cellular Localization |
Membrane
|
| Concentration |
≥ 3 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Christine Guntermann, et al. JCI Insight. 2017 Mar 9;2(5):e91127. Tong Du, et al. Front Immunol. 2020 Jul 23:11:1603. |
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